Expertise
Imaging Platforms
Keywords - Electron Microscopy: Transmission EM | Scanning Transmission EM | Electron tomography | Scanning EM (FEG) | Serial Block Face SEM | Cryo-TEM | Cryo-ET
1) Thermo Fisher Glacios 200kV FEG transmission electron microscope with Falcon 3EC direct electron detector
2) Thermo Fisher Talos L120C transmission electron microscope with Ceta high speed camera and STEM detectors
3) FEI Tecnai 12 Biotwin transmission electron microscope with Gatan Orius SC1000 CCD camera
4) FEI Quanta 250 FEG scanning electron microscope with Gatan 3View attachment for serial block face imaging
Applications
Keywords - Biological: Cell Biology | Neurobiology | Developmental Biology | Plant Biology | Zebrafish | Drosophila | Bio-materials
The facility is actively involved in a wide range of research topics across the Faculty and in the past few years has contributed to significant number of scientific publications in high profile biological journals, including publications where electron microscopy staff members are authors.
Sample Preparation
Keywords - Biological: Resin embedding | Ultrathin sectioning | Serial sectioning | Tokuyasu embedding | Cryosectioning | Immunolabelling | Plunge Freezing | High Pressure Freezing | Freeze substitution | CEMOVIS | Critical Point Drying | Freeze fracture | Metal coating | Negative stain | Rotary shadowing | Correlative Light & EM | PLT
- Baltec HPM010 high pressure freezer with Leica AFS2 freeze substitution unit
- Leica UCT, UC6, UC7 ultramicrotomes and cryoultramicrotomes
- Miscellaneous TEM, cryo TEM and SEM sample preparation equipment.
- Computer station with Wacom tracing pad for 3D rendering.
- Linux workstations for single particle analysis.