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Experimental Medicine Division/Translational Gastroenterology Unit, University of Oxford

The EMD/TGU flow cytometry facility is based in the John Radcliffe Hospital, Oxford, and offers a high-quality cell sorting and analysis service. The facility has a LSRII SORP and LSRFortessa analysers, a Cytek Aurora spectral cytometer and a FACSAriaIII cell sorter. All users are fully trained to use the analysers and we offer advice on all aspects of flow cytometry experiments, including sample preparation and panel design. We welcome users from both within and outside of the University of Oxford



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Expertise

Platforms

Keywords - Platforms: Analytical Flow Cytometry | Cell sorting 
  • LSRII, with HTS plate reader: 405nm, 488nm, 532nm, 640nm - 18 fluorescent parameters
  • LSRFortessa: 405nm, 488nm, 640nm - 14 fluorescent parameters
  • AriaIII: 405nm, 488nm, 561nm, 640nm - 17 fluorescent parameters
  • Cytek Aurora: 335nm, 405nm, 488nm, 561nm, 640nm

Applications

Keywords - Applications: Phenotyping | Cell cycle | Mammalian cells | Fluorescent proteins | Functional studies 

Data Analysis

Keywords - Software: FlowJo 

Members of the University can be registered to our FlowJo site-license for an annual fee.

Shared Access

The facility is open members of the University and also, if appropriate, to external users from Academia or Industry. 

Funding

The facility operates under a Full Economic Costing model. 



Point of Contact

Experimental Medicine Division/Translational Gastroenterology Unit, University of Oxford
Room 5605, Level 5
John Radcliffe Hospital
Headington
Oxford, OX3 9DU , United Kingdom


Point of Contact

Dr Helen Ferry  01865228964