Expertise
Imaging Platforms
Keywords - Light Microscopy: Superresolution microscopy, Live cell imaging, PALM, Light-sheet microscopy (SPIM), Frequency-domain FLIM, Confocal microscopy, Widefield microscopy, TIRF, Multi-photon microscopy, STORM, FCS/FCCS, Structured Illumination Microscopy
Keywords - X Ray Microscopy: MicroCT
Applications
Keywords - Biological: Cell Biology, Developmental Biology, Plant Biology, C.elegans, Microbiology, Histology/Pathology, Neurobiology, Immunobiology, Zebrafish, Drosophila | High content screening as
We support a wide range of platforms. As well as standard confocal and widefield we host SIM and STORM super-resolution platforms. We also support Slide scanning, FCS, FRET, FRAP-PA, Tau FLIM and MicroCT. We have a home build multiphoton-SRS Raman sytems.
Sample Preparation
Keywords - Biological: Immunofluorescence, H&E staining
Mostly our facility users prepare their own samples, however we are happy to provide advice as required.
Data Analysis
Keywords - Software: ImageJ | FIJI | Imaris | Amira | Matlab | Huygens | Volocity | Metamorph | OMERO
Keywords - Hardware: We provide in person and remote access to our BioImage analysis resources
The AIR has a Image Informatician and staff members who can provide advice and a bespoke scripting service as well as support with image analysis.
Shared Access
Our facility is open to all. Please see here for more information: https://www.ed.ac.uk/institute-genetics-cancer/facilities/advanced-imaging-resource
We also house the University of Edinburgh ESRIC facilities: http://www.rms.org.uk/outreach/index/facilities-database-2/facdatESRIC
Funding Overview
The AIR receives funding from a variety of sources. The MRC-HGU imaging facilities are mostly funded through the MRC. ESRIC is funded from a MRC next generation optical microscopy award. Wellcome, CRUK, ERC, BBSRC also provide substantial funding for imaging as do UK charities