Expertise
Imaging Platforms
KEYWORDS - ELECTRON MICROSCOPY: Transmission EM, Electron tomography, Scanning EM (FEG), Integrated Light and SEM, Scanning Transmission EM, Serial Block Face SEM, Integrated Light and TEM
JEOL JEM-1400 TEM – EMSIS Xarosa digital camera
ZEISS GEMINI 350 FEG-SEM - SmartSEM, ZEN and ATLAS software
Applications
KEYWORDS - BIOLOGICAL: Cell Biology, Developmental Biology, Plant Biology, C.elegans, Microbiology, Histology/Pathology, Neurobiology, Zebrafish, Drosophila, Bio-materials, Food
KEYWORDS - PHYSICAL SCIENCES: BIOENGINEERING & BIOMATERIALS | NANOMATERIALS
Being a core facility, we have experience with a broad range of materials and research topics, including ultrastructure and morphology of animal and plant tissue, drug development and delivery, cell culture, micro-organisms and biofilms, and proteins and macromolecules. Depending on researchers needs, we can provide a complete sample preparation and imaging package, or training and access to our user friendly microscopes.
Sample Preparation
KEYWORDS - BIOLOGICAL: Resin embedding, Serial sectioning, Critical point drying, Metal coating, Correlative Light & EM, Ultrathin sectioning, Immunolabelling, Negative stain
- Leica EM UC7 ultramicrotome
- Balzers Critical Point Dryer
- Quorum Q150TES - sputter coating, glow discharging, carbon evaporation attachments
- Quorum Gloqube
- Array tomography preparation and aquisition
- Single Particle Analysis negative staining
- Immuno-labelling
- Correlative Light and Electron Microscopy (CLEM) - In collaboration with the UoL Advanced Imaging Facility (AIF)
Shared Access
The EMF is an open access facility within the University of Leicester Core Biotechnology Services. We welcome academic and commercial projects from outside the University. Please contact us for further details and access charges.
Funding
The maintenance of the facility is funded by usage charges.