Expertise
Imaging Platform
Keywords - Electron Microscopy: Transmission EM | Electron tomography | Scanning EM (FEG) | Focused Ion Beam SEM | Cryo-ET | Cryo-TEM
Instruments include FIB-SEMs for milling/correlative experiments bacteria/cells/organoids/tissues (Aquilos2 FIB/SEM, Arctis pFIB/SEM), TEMs for screening/high-resolution single particle/tomography data collection (Glacios, Krios G3i with a Falcon-4i/SelectrisX detector) as well as ancillary equipment for grid preparation (micropatterning for cell placement, plunge freezing).
Applications
Keywords - Biological: Cell Biology | Immunobiology | Virology
We have the unique capability of preparing/collecting data on BSL3 viruses.
Sample Preparations
Keywords - Biological: Plunge freezing | Correlative Light & EM | High pressure freezing | Negative stain
Keywords - Materials: Argon ion thinning | Focused ion beam (Gallium) | Focused ion beam (other)
We have the unique capability of preparing BSL3 virus samples and are open to helping with preparation of any samples for cryoTEM, including for materials sciences.
Data Analysis
Keywords - Software: IMOD
Keywords - Hardware: TEM | SEM | pFIB | FIB-SEM | Vitrification | HPF
Funding Overview
Access to our facility is available at request from academia and industry alike, and funding is available via Instruct.
We provide support and advice for both short term projects (including generating preliminary data for grant applications) and can provide training for longer term use.