Expertise
Imaging Platforms
Keywords - Light Microscopy: Superresolution microscopy | Live cell imaging | PALM | Confocal microscopy | Widefield microscopy | TIRF | STORM | Structured Illumination Microscopy
There are 4 systems that can be temperature and atmospherically controlled (down to microaerophillic conditions)
*DeltaVision OMX Flex (4 cameras): BF, WF, Line Scanning Confocal, 3D-SIM, Ring-TIRF, STORM/PALM.
*Deltavision OMX Flex (3 cameras): BF, WF, Line Scanning Confocal, 3D-SIM, Ring-TIRF, STORM/PALM.
*Molecular Devices Image Xpress µConfocal: BF, WF, Spinning Disk Confocal.
*Zeiss LSM880 upright confocal microscope with AiryScan: BF, WF, Pinhole Confocal, AiryScan Super Resolution, Spectral Unmixing.
*DeltaVision Elite (with 96 well Stage and live-cell imaging filter sets): BF, WF.
We also have:
*Olympus VS200 Slide Scanner: BF, WF, AutoOiler, C
*Zeiss AxioImager M2: BF, WF.
*Olympus BX53: BF, WF.
*Leica M102S Stereoscope: BF.
All equipment is housed in a CL2 laboratory.
Applications
Keywords - Biological: Cell Biology | Plant Biology | Microbiology | Immunobiology | Food
Sample Preparations
Keywords - Biological: Resin embedding | Serial sectioning | Cryosectioning | Immunofluorescence | Vibrating microtome | Enzyme histochemistry | Ultrathin sectioning | Immunolabelling | Negative stain | Cryostat sectioning | Live cell
We have a fully functional histology laboratory. We also have an electron microscopy sample preparation service. There are dedicated sample preparation facilities for external workers and access to cell culture, CL3 and anaerobic microbiology facilities.
Data Analysis
Keywords - Software: ImageJ | Imaris | ImagePro | Fiji
Other: InCarta
Keywords - Hardware: We have three dedicated computers running with nVIDIA Quadro RTX8000 Graphics Cards and and Intel i9 processors for the most advanced machine learning analysis
Access can be through partnership with academics at the Quadram Institute BioScience or through the commercial arm of the organisation QIB XTRA. Charges are determined by negotiation.
Funding Overview
We are a BBSRC funded core facility.