Expertise
Platforms
Keywords - Platforms: Analytical Flow Cytometry | Cell sorting | Imaging Flow Cytometry
The facility is equipped with 2 cell sorters and 3 analysis flow cytometers:
- Sorters: FACS Aria IIu cell sorter and BDFACS Discover S8 (spectral and imaging).
- Flow cytometry analysers: BD Canto II (3 lasers), BD LSR Fortessa (4 laser) and Sony ID7000 (spectral)
Cell sorting is performed as an assisted service by the facility staff, and analysis is generally performed by users after comprehensive training.
Applications
Keywords - Applications: Phenotyping | Cell cycle | Mammalian cells | Plant biology | Yeast biology | Bacteria | Fluorescent proteins | Functional studies | Clinical studies
Other: Algae | Trypanosome
Due to the diverse user-base, our staff are highly experienced in many flow cytometry and cell sorting applications.
Data Analysis
Keywords - Software: Flowjo
Other: ID7000 system software
The facility has a workstation available to book for FlowJo analysis, and there are some FlowJo dongles available for use.
Staff offer software training and are available to support data analysis and interpretation.
Shared Access Overview
We are accessible to both University of Edinburgh and external users. Please contact the facility for more information.
Funding Overview
The facility operates through a re-charge/cost recovery model. Please contact the facility for more information on charges.