The Pirbright Institute Flow Cytometry Facility

The Pirbright Institute is a national virus research facility specifically designed for the study and diagnosis of veterinary and zoonotic pathogens which are exotic to the UK. The Flow Cytometry facility provides researchers with state-of-the-art instrumentation, cell sorting services, high-quality training and expertise within low and high containment environments. We specialise in sample preparation, acquisition, sorting and analysis of biological samples from farm animals.



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Expertise

Flow Cytometry Platforms

Keywords - Light Microscopy: Analytical Flow Cytometry | Cell sorting 
 

The Plowright building, High containment facility (SAPO4/BSL-2)
1x 5-laser Cytek Aurora+ APD+ ASL (UV/V/B/YG/R)
1 x 4-laser BD LSR Fortessa + HTS (V/R/YG/B)
1 x 4-laser BD FACSAria Fusion (V/R/YG/B)
1 x 3-laser Miltenyi MACSQuant 10 (V/R/B)
1 x 3-laser Miltenyi MACSQuant 16 (V/R/B)


The Jenner building, Low containment facility (BSL-2)
1x 5-laser Cytek Aurora+ APD+ ASL (UV/V/B/YG/R)
1x5-laser Thermo Bigfoot (UV/V/B/YG/R)
1 x 3-laser Miltenyi MACSQuant 10 (V/R/B)
1 x 3-laser Miltenyi MACSQuant 16 (V/R/B)
1 x 4-laser BD LSR Fortessa + HTS (V/R/YG/B)
1 x 4-laser BD FACSAria UIII (V/R/YG/B)

Applications 

Keywords - Applications: Phenotyping | Cell cycle | Mammalian cells | Fluorescent proteins | Functional studies | Microvesicles | Screening (high throughput) 

The facility is used for a range of applications including immunophenotyping, functional assays and high-throughput screening (HTS).

Data Analysis

Keywords - Software: FlowJo | FCS express

The facility provides users with access to Flowjo, FCSExpress, BD FACS DIVA and MACSQuantify

Shared Access 

The facility welcomes all Pirbright Institute members, and external users. 

Funding Overview 

The Pirbright Institute receives strategic funding from BBSRC

 



Point of Contact

The Pirbright Institute Flow Cytometry Facility
Flow Cytometry at The Pirbright Institute
The Pirbright Institute
Ash Road
Pirbright, Surrey, GU24 0NF, United Kingdom


Point of Contact

Katy Moffat 01483 231259

Staff List

Katy Moffat
Chris Chiu