Virtual 12th Light Sheet Fluorescence Microscopy Conference 2020

23 – 25 September 2020

Online

RMS Hosted Event

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Speakers


Talk Title: Holistic Three-dimensional Cellular Mapping of Mammalian Organs by Tissue Clearing and Light-sheet Fluorescence Microscopy 

  • Shan Zhao

    Helmholtz Zentrum München
    Shan Zhao is a PhD researcher in Institute for Tissue Engineering and Regenerative Medicine (iTERM) of Helmholtz Zentrum München and LMU. Shan did the bachelor in Pharmaceutical Engineering & Technology and the master in Applied Chemistry in Beijing Institute of Technology, China. Shan developed tissue labeling, clearing and imaging technologies to study structural details of tissue at cellular level across whole mammalian organs including mouse, pig and human.


Talk Title:  How to define and optimize axial resolution in light-sheet microscopy: a simulation-based approach

  • Elena Remacha

    IGBMC / Leica Microsystems
    Elena Remacha is a PhD student working in a joint project for the Institute of Genetics and Molecular and Cellular Biology (IGBMC) in Strasbourg and for Leica Microsystems. She received her BSc in Biomedical Engineering and her MSc in Industrial Mathematics in the University Carlos III, Madrid. Her work is aimed at optimizing light sheet-microscopy for cardiovascular research in the zebrafish embryo.

 


Talk Title: Physical expansion of cells and tissues: Development and application to light-sheet fluorescence microscopy   

  • Ruixuan Gao

    MIT / HHMI Janelia, United States
    Ruixuan Gao is a postdoctoral associate in the Synthetic Neurobiology Lab at MIT and a visiting postdoctoral associate at HHMI Janelia Research Campus. His research lies at the cross-section of chemistry and biological sciences, with a primary focus on nanoscale imaging and sensing of cells and tissues. In particular, his research interests span rational design of macromolecular matrices, supramolecular assemblies, and chemical linkers/probes to retain and visualize endogenous biomolecules in cells and tissues, as well as development of microscopy modalities to map and track these molecular entities across multiple spatial and temporal scales. Some of Ruixuan’s recent works include a homogenous hydrogel matrix that physically magnifies biological specimens with nanoscopic accuracy, and a scalable fluorescence imaging pipeline that captures cellular connections and molecular compositions in the brain at nanoscopic resolution. Ruixuan received his Ph.D. in Chemistry from Harvard University.


Talk Title:  Custom light sheet microscopy developments and applications in an imaging  facility environment

  • Martin Lenz

    CAIC Cambridge
    Martin O. Lenz is Senior Research Associate at the University of Cambridge with 17 years of experience designing bespoke optical instruments and more than 12 years developing high-end optical microscopes. He worked at some of the world’s leading groups for application of super-resolution microscopy (P. French, Imperial College London) and IINS Bordeaux where he successfully implemented point-scanning STED super-resolution microscopy with single molecule based super-resolution on the same microscope platform. In recent years he has successfully developed multiple light sheet microscopes for application in biological sciences at the Cambridge Advanced Imaging Centre. His expertise centers around hardware and software development for advanced light microscopy and integrating new and existing technologies for successfully pushing the boundaries of microscopy.


Talk Title: Hybrid open-top light-sheet microscopy for multi-scale imaging of cleared tissues 

  • Adam Glaser

    University of Washington
    Adam Glaser is an Instructor in Mechanical Engineering at the University of Washington and recent recipient of a Pathway to Independence early career award from the NIH/NCI. He received his B.S. in Mechanical Engineering from Tufts University in 2010 and his Ph.D. in Engineering Sciences from Dartmouth College in 2015. Through a fellowship from the NIH/NCI, he completed his postdoctoral research at the University of Washington from 2015 – 2019 in Dr. Jonathan Liu's laboratory. His work is now being commercialized by and has led to the co-founding of the start-up companies Dose Optics and Lightspeed Bio.


Talk Title:  LSFM underscores the variability and complexity of yolk microtubule organization during early zebrafish development

  • MarĂ­a Marsal

    SLN ICFO Barcelona
    Maria Marsal is a postdoctoral researcher at ICFO (Institute of Photonic Sciences) in the Super Resolution Light Microscopy and Nanoscopy lab (SLN). She received her Ph.D. in Biology from Barcelona University. She is a developmental biologist by training, both in the fields of regeneration and development with a special focus on zebrafish early embryogenesis, and a microscopy specialist. She has a broad expertise in light microscopy, from classical laser scanning confocal microscopy up to super resolution and light sheet fluorescence microscopy techniques, with national and international collaborators, within European networks (Euro Bioimaging, CORBEL, Laser Lab-Europe). She was recently awarded with a BIST (Barcelona Institute for Science and Technology) To the Mothers of Science grant. Her research work currently focuses in the global analysis of the yolk microtubule cytoskeleton of the zebrafish across multiple temporal and spatial resolutions. 


Talk Title: High-speed in-vivo 3D microscopy across scales 

  • Professor Elizabeth Hillman

    Columbia University
    Elizabeth Hillman is a Professor of Biomedical Engineering and Radiology and a member of the Zuckerman Mind Brain Behavior Institute at Columbia University. She received her undergraduate and PhD training in Physics at University College London and completed post-doctoral training at Massachusetts General Hospital, Harvard Medical School. She has developed a wide range of optical imaging and microscopy technologies to capture dynamic in-vivo function at high speeds, applying them to studies of brain function and physiology in species ranging from C. elegans worms and Drosophila to awake, behaving mice.


Talk Title: eSPIM: Light-sheet microscopy for cell biology

  • Bo Huang

    UCSF San Francisco
    Dr. Bo Huang is a Professor of Pharmaceutical Chemistry and of Biochemistry and Biophysics (joint) at University of California, San Francisco, and a Chan Zuckerberg Biohub Investigator. He received his B.S. degree in Chemistry from Peking University, China, in 2001 and Ph. D. degree in Chemistry at Stanford University in 2006. After finishing postdoc work at Harvard University in 2009, he joined UCSF as an Assistant Professor. He was promoted to Associate Professor in 2014 and Professor in 2017. Dr. Huang’s research work encompasses the areas of optical microscopy, bioengineering, biophysics and cell biology. Dr. Huang has received many awards, including the GE Healthcare and Science Prize for Young Life Scientists, Searle Scholarship, Packard Fellowship for Science and Engineering, the NIH Director’s New Innovator Award, the American Society for Cell Biology Young Life Scientist Award, and UCSF Byers Award for Basic Science.


Talk Title: Visualizing and quantifying cell behaviors and states across scales in mammalian embryos 

  • Kat Hadjantonakis

    Sloan Kettering Institute
    Developmental and stem cell biologist Anna-Katerina (Kat) Hadjantonakis is Chair of the Developmental Biology Program at the Sloan Kettering Institute of Memorial Sloan Kettering Cancer Center in New York. Her lab seeks to understand how individual cells regulate their identity, and how they work together to reproducibly build complex organs in mammalian embryos, and in vitro stem cell and organoid models. Her lab employ a suite of quantitative analysis approaches including microscopic imaging.  


Talk Title: Fishing for leukocytes: Cellular and subcellular dynamics of migrating immune cells revealed by in vivo lattice light-sheet microscopy 

  • Harriet Manley

    Australian Regenerative Medicine Institute / Monash University
    Harriet Manley is a postdoctoral research fellow at the Australian Regenerative Medicine Institute (ARMI) with the Lieschke group, which exploits the optical transparency of zebrafish larvae to examine leukocyte biology and behaviour in vivo. Her research focuses on the application of intravital lattice light-sheet microscopy (LLSM) to visualize cellular and subcellular morphodynamics during rapid immune cell migration. For this work, she has collaborated the Advanced Imaging Center at HHMI Janelia Research Campus and Monash Micro Imaging to use both Betzig and prototype 3i LLSM systems, and has been awarded an Australian Microscopy & Microanalysis Society bursary to present at the 19th International Microscopy Congress. Capturing the in vivo subcellular dynamics of the fastest migratory cell type, neutrophils, via LLSM represents a technological feat that has recently been published and highlighted in J. Leuk. Biol. Her current research investigates the nuclear envelope plasticity and polarity of migrating neutrophils in vivo.


Talk Title:  Light Sheet Microscopy Coupled to an Atomic Force Microscope to Study the Engulfment Forces During Phagocytosis

  • Megan Kern

    University of North Carolina
    Megan Kern is a PhD researcher in the Richard Superfine lab at the University of North Carolina - Chapel Hill. She is interested in how cells interact with their environment and generate force. To study this, she uses a unique system that combines Atomic Force Microscopy with Line-Bessel Light sheet microscopy to study mechanical response at the single-cell level. Specifically, this system allows high-resolution, volumetric imaging of the actin cytoskeleton with time-correlated engulfment force measurements of individual macrophages during phagocytosis.


Talk Title:  How biofilms form and single cells flow: insights revealed by light sheet microscopy

  • Boyang Qin

    Princeton University
    Boyang Qin is a postdoctoral research associate at Princeton University, with a joint appointment in the Departments of Molecular Biology and Mechanical and Aerospace Engineering. His research themes lie at the interface of biophysics, mechanics, and biological sciences. In particular, his research interests include adapting light sheet microscopy and developing other imaging modalities to the study of cellular dynamics and morphogenesis during bacterial biofilm development and the study of the mechanics of soft biomaterials. Some of Boyang’s recent works include tracking single cell positions and lineages during bacterial biofilm development using dual view light sheet microscopy, mapping the dynamics and mechanics of bacterial pellicle morphogenesis, and studying the mechanical response of ciliary beating in mucus-like viscoelastic fluid environments. Boyang received his Ph.D. in Mechanical Engineering and Applied Mechanics from University of Pennsylvania.


  • Loic Royer


 Talk Title: On Flow and Form: Building tools to visualise morphogenesis

  • Nico Scherf

    TU Dresden
    I studied Computer Science and Biomedicine at the University of Leipzig and did my PhD at the TU Dresden in Bioinformatics and Medical Biometry. I was a PostDoc in the lab of Jan Huisken at the MPI CBG and then at the Department of Neurophysics at the MPI CBS in Leipzig. Currently I lead the research group “Machine Learning in Biomedical Informatics” at the TU Dresden.


Talk Title: Tips for adapting to LISH image visualization and analysis 

  • Holly C. Gibbs

    Texas A&M University
    We have yet to understand how embryonic brain development gives rise to brain function and how deficits in this process lead to brain disorders. To help improve this understanding, Dr. Holly Gibbs is interested in developing accessible microscopy, labeling, visualization, and bioimage informatics tools for creating multi-scale models of the emergence of brain structure and function. She earned her Ph.D. in Biomedical Engineering from Texas A&M University in 2014 studying neurodevelopment in zebrafish using multi-photon microscopy. In March 2019, she joined the Microscopy and Imaging Center at Texas A&M University, where she is currently funded as an Imaging Scientist by the Chan Zuckerburg Initiative. This grant program aims to provide imaging cores with support for accelerating the adoption of novel imaging techniques and open source processing software at their institutions.


 Talk Title: Illuminating arthropod development with light-sheet microscopy

  • Tassos Pavlopoulos

    Institute of Molecular Biology and Biotechnology (IMBB-FORTH)
    Anastasios (Tassos) Pavlopoulos is a group leader at the Institute of Molecular Biology and Biotechnology (IMBB-FORTH) in Crete, Greece. He is a developmental biologist by training interested in the molecular, cellular and mechanical basis of tissue and organ morphogenesis during animal development and evolution. To achieve these goals, his lab is studying and comparing development of genetically and optically tractable arthropod species using various approaches, including light-sheet imaging and tracking. His research benefits enormously from longstanding and enjoyable collaborative interactions with the Tomancak group at MPI-CBG (Dresden, Germany), the Keller group at HHMI Janelia Research Campus (Ashburn, USA), the Averof group at IGFL (Lyon, France) and others.


Scientific Organisers

  • Emmanuel G. Reynaud

    UC Dublin
    Emmanuel, alongside Pavel Tomancak has been pushing the Light Sheet Microscopy Community forward organizing EMBO courses, workshops and conferences. He is a Lecturer in Cell Biology at University College Dublin with a keen interest in 3D imaging on land but also underwater. He has been supporting companies such as Carl Zeiss Microscopy, Luxendo as well as NGOs to get the message across!

  • Chris Dunsby

    IC London
    Chris Dunsby is a Reader at Imperial College London with a joint appointment between the Department of Physics in the Faculty of Natural Sciences and the Department of Medicine in the Faculty of Medicine. His research interests include LSFM, advanced quantitative microscopy techniques and ultrasound super-resolution imaging.

  • Kate McDole

    MRC LMB Cambridge
    Kate McDole is a newly appointed Group Leader at the MRC Laboratory of Molecular Biology in Cambridge, UK. Her lab explores the morphogenesis of the early mouse embryo using a combination of advanced light-sheet microscopy, biology, computational methods and biophysics.

  • Gopi Shah

    EMBL Barcelona
    Gopi Shah is the Project Manager for Advanced Mesoscopy Applications at the Mesoscopic Imaging Facility (MIF) at EMBL Barcelona. Her current scientific work focuses on establishing live imaging of embryonic and in vitro 3D models of animal development and diseases in collaboration with labs at EMBL and beyond. Broadly, she is interested in designing imaging infrastructures that enable scientists to seamlessly acquire, analyse and visualise large-scale imaging data.

  • Montserrat Coll

    EMBL Barcelona
    Montserrat Coll is an imaging scientist at the Mesoscopic Imaging Facility (MIF) at EMBL Barcelona. She is a biologist with research experience in developmental biology and a PhD on marine microbial ecology. Currently she works exploring different clearing methods applied to a broad range of tissues, organs and organisms. She likes to bring small details to light. 

  • Robert Haase

    CSBD / MPI CBG Dresden
    Robert Haase is a computer scientist by training and follows his curiosity deeper and deeper into the life sciences. He is associate lecturer for bio-image analysis, bio-statistics and programming at the Biotechnology Center of the TU Dresden. In his postdoctoral research in Gene Myers lab at the Center for Systems Biology and the Max Planck Institute for Molecular Cell Biology and Genetics in Dresden he studies Tribolium castaneum embryo development using smart light sheet microscopes, real-time image analysis and machine learning exploring new ways how scientists interact with multi-dimensional image data to gain new insights.

  • Reto Fiolka

    UT Southwestern
    Reto Fiolka is a mechanical engineer by training and his research aims to extend the number of biological questions that can be answered with light microscopy. He is an assistant Professor at UT Southwestern Medical Center, where his microscopes are used in interdisciplinary research programs. The microscope development in his lab is focused on improving the spatial and temporal resolution, reducing light dosage and improving the optical penetration depth. These technological improvements are particularly critical for the study of cellular dynamics and signalling pathways involved in cancer growth and metastasis in three-dimensional environments. The challenge to volumetrically image such processes with sufficient temporal and spatial resolution and over relevant length and time scales made light-sheet microscopy an obvious choice in the Fiolka lab.  

  • Deirdre Kavanagh

    University of Birmingham
    Deirdre Kavanagh is an imaging scientist and facility manager working at the Centre of Membrane Proteins and Receptors (COMPARE), University of Birmingham. She has expertise in fluorescence microscopy with specialist interest in super-resolution, fluorescence correlation spectroscopy and light-sheet technologies. She completed a PhD in Engineering and Physical Sciences and as post-doctoral researcher she applied advanced microscopy to investigate the molecular mechanisms underlying cell communication. At COMPARE, she supports a large user base via teaching, training and assisted imaging and analysis sessions. She is proactively involved in the organisation and development of microscopy networks, workshops, courses and public engagement. She serves on a number of microscopy committees including the Royal Microscopical Society Light Microscopy Committee, and she co-founded the UK’s Lattice Light-Sheet community group.
     



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