infocus #55 September 2019 Multiphoton imaging in Arthritis Research

This review will focus on rapid advances in illumination sources such as pulsed infrared lasers for multiphoton imaging and near-infrared fluorescent (NIRF) probes that promise to overcome these obstacles, enabling analysis of cellular and molecular events in vivo in a non, or minimally, invasive manner.

DOI: 10.22443/rms.inf.1.178

Rheumatoid arthritis (RA) is a prevalent autoimmune disease which primarily affects the joints of the hands and feet, however, it is also associated with significant co-morbidities including cardiovascular disease and cancer. Optical imaging technique is a major approach employed in in vivo RA research due to its advantages over other bio-imaging modalities such as MRI, radiography and ultrasound, specifically its sensitivity and cellular resolution. However, key limitations remain with this technology, particularly the limited depth of imaging and problems due to tissue autofluorescence.

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Authors

Ashleigh Sayer

Ashleigh Sayer is a recent graduate from the master’s programme in Immunology and Inflammatory Disease at the University of Glasgow. She is currently undertaking the NHS Scientist Training Programme specialising in Clinical Immunology at Cambridge University Hospitals and is pursuing a second master’s degree in Clinical Blood Sciences at the University of Manchester.

Robert A Benson

Research Associate, Institute of Infection, Immunity & Inflammation University of Glasgow

Dr Robert Benson is a Research Associate at the Institute of Infection, Immunity and Inflammation, University of Glasgow. His research interests lie in understanding dynamic cellular processes, using advanced imaging techniques to visualise complex in vivo protective and pathological immune responses

James M Brewer

Institute of Infection, Immunity & Inflammation University of Glasgow

James Brewer is the Professor of Basic Immunology in the Institute of Infection, Immunity and Inflammation, University of Glasgow. His research applies advanced imaging techniques to enhance our understanding of the basic biology of the immune system in real time, in vivo.