infocus #68 December 2022 Working smarter with optical filters and LED illumination

Microscopy experts are well versed in the importance of carefully matching the fluorophore selection, optical filters and light source for fluorescence microscopy experiments.


However, the advancement of LED illumination technology for widefield fluorescence has introduced new and sometimes surprising opportunities for high-speed, high-contrast multilabel imaging. Below we cover several options which range in complexity, cost and performance – and also provide advice to keep in mind when using optical filters with LED illumination.