High content imaging (HCI) involves automated collection and analysis of images to provide quantitative data on a range of functional and morphological changes in cells, spheroids or organoids. It has become a widely used tool in cell biology and drug discovery, for example in screening of drug candidates, fragments, RNAi, miR and phenotypic screens using CRISPR-Cas9, amongst other. This meeting will introduce the power of HCI to new or inexperienced users, but will also cover current HCI case studies from leaders in the field and advances in HCI techniques and analysis. Major suppliers of HCI software and analysis will be present to demonstrate applications and uses of their HCI equipment and software. Anyone interested in HCI, whether starting out in the field or an experienced user, will benefit from attending
University of Nottingham
Steve is a Principal Research Fellow in the Institute of Cell Signalling, School of Biomedical Sciences. His interest is in using imaging approaches to understand the molecular pharmacology and organisation of G-protein coupled receptors. These are a large family of cell surface proteins, which are targets for many currently used drugs. With a background in pharmacology and cell signalling, Steve’s focus since arriving in Nottingham in 2000 has been on applying microscopical techniques, such as fluorescence correlation spectroscopy, confocal and wide-field fluorescence microscopy and TIRF, to study how GPCRs are compartmentalised in the cell membrane, and how this affects their pharmacology.
University of Leeds
Jacquie is a Senior Lecturer in the Leeds Institute of Biomedical and Clinical Sciences, Faculty of Medicine and Health based at St James’s University Hospital. Her research group uses imaging approaches to investigate genes and proteins involved in mitosis, which when mutated cause Autosomal Recessive Primary Microcephaly (MCPH). The group use live cell imaging, confocal, super resolution and high-content high-throughput microscopy to identify and quantitate changes in mitotic spindle orientation, microtubule and actin organisation and cell cycle progression in patient cells and modified cancer cells. Jacquie’s interest in cell biology and imaging has led to her developing a high-throughput high-content imaging bio-screening facility at Leeds, which screens whole and partial genome siRNA/miRNA libraries and small molecule libraries to identify components of biological/disease pathways, therapeutic targets and novel therapeutic drugs. Currently she is the Academic Lead for imaging for the SCIF Flow Cytometry and Imaging Facility, University of Leeds, which for imaging encompasses a number of widefield, live cell and confocal imaging systems and the bio-screening service.
Sam is an Associate Principal Scientist currently working within the Functional Genomics team at AstraZeneca in Cambridge, UK. My current role is centred around the use of genetic (including CRISPR reagents) and other perturbants to interrogate complex biology and identify new targets for the treatment of different diseases. I have a particular interest in the application of confocal fluorescent microscopy to develop high content, phenotypic cell & tissue based imaging assays for target identification and validation. Before joining AstraZeneca in 2010, I held a postdoctoral research position at the University of Nottingham where my work focussed on cell signalling in airway smooth muscle. I have a PhD in cell signalling from the University of Nottingham and a BSc in Pharmacology from the University of Bath.
Head of SLIM - School of Life Sciences Imaging Facility, Faculty of Medicine & Health Sciences
Tim Self is a Chief Experimental Officer and Head of the Imaging facility at the School of Life Sciences, University of Nottingham. His interest and passion for microscopy started in his undergraduate project using electron microscopy at the University of Sheffield and has spent the proceeding 35 years putting that passion into practice. For half of his career Tim has applied electron microscopy to many biological research questions until in 2000 he saw the light and moved over full time to light microscopy. He now runs a large core facility with EM, histology, super resolution, confocal, wide field, High Content, FCS and TIRF imaging at the University of Nottingham.