As part of the Imaging ONEWORLD series, the focus of these lectures is on microscopy and image analysis methods and how to apply these to your research. Almost all aspects of imaging such as sample preparation, labelling strategies, experimental workflows, ‘how-to’ image and analyse, as well as facilitating collaborations and inspiring new scientific ideas will be covered. Speakers will be available for questions and answers. The organisers, CRUK CI core facility staff, Gurdon Institute, MRC-LMB, MRC Cancer Unit and NPL will be able to continue the discussion and provide advice on your imaging projects.
Scientific Organisers
Dr Kirti Prakash FRMS
Institute for Cancer Research (ICR) and Royal Marsden Trust
Kirti Prakash is a computer scientist by training (Bachelors and Masters degree) but a biologist at heart (PhD degree). Kirti aspires to be an inventor and develop new imaging tools for cell biology and neuroscience. Kirti did his Masters in Computer Science from Aalto University (Finland) and PhD in Biology from Heidelberg University (Germany). During his PhD, he developed a new method to image DNA which led to the first high-resolution images of the epigenetic landscape of meiotic chromosomes and mechanisms behind chromosome condensation. The doctoral research earned him several awards including Springer Best PhD Thesis Prize. After his PhD, he did a couple of postdocs at Carnegie Institution for Science (USA) and University of Cambridge (UK). The primary highlights of his research here were laser-free superresolution microscopy and development of a high-content imaging pipeline to quantify single-cell gene expression. Formerly at the National Physical Laboratory (NPL), and currently working at the Institute for Cancer Research (ICR) and Royal Marsden Trust, he is working on microscope development and image analysis.
Dr Alex Sossick
Alex heads the Imaging Facility at the Gurdon Institute, which includes a variety of microscopy techniques including confocal, high throughput and deconvolution. He is keen to raise the level of microscopy understanding and application, and runs and takes part in various microscopy courses.
Dr Alessandro Esposito
MRC Cancer Cell Unit
Alessandro’s major focus is to develop new methods for probing and mapping the biochemical events that underlie cellular systems using fluorescence microscopy, with the overall aim of better understanding the fundamental principles that govern tumour suppressive mechanisms and their perturbation in cancer. In his career, Alessandro has developed several technologies (e.g. high throughput FLIM, solid state detectors and imaging specotrpolarimetry) and methods of analysis for quantitative biochemical imaging (FLIM, spectral imaging and anisotropy imaging). Since 2009, he has focused on strategies to enhance the biochemical resolution of fluorescence microscopy and multiplexing several biochemical reactions within the cell with the scope to correlate complex biochemical signatures to cell fate decision and homeostatic control.
Dr Stefanie Reichelt
CRUK Cambridge Institute
Stefanie Reichelt, PhD is head of the light microscopy facility at the CRUK Cambridge Institute. The core provides state-of-the-art imaging resources, training courses for scientists and students and develop new imaging systems as well as user-friendly analysis and acquisition tools for specific research applications. Stefanie teaches academically at Cambridge University, in scientific workshops and out-reach events. She is also founder of the well-known Plymouth Advanced Microscopy Course and the Centre for Mathematical Imaging in Healthcare (CIMH)
- Email:
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Correlative Light and Electron Microscopy (CLEM): Combining Ultrastructural and Molecular Imaging
Electron microscopy (EM) is the method of choice for imaging biological samples at the ultra-structural level. Yet, with notable exceptions, its molecular contrast is limited. Fluorescence light microscopy (LM), on the other hand, provides exquisite biomolecular specificity; however, its resolution (even in the case of super-resolution imaging) typically lags behind EM. Therefore, a number of methodologies have been developed to combine the advantages of both imaging modalities, termed Correlative Light and Electron Microscopy (CLEM). This talk will briefly outline major efforts in this area, and will focus on developments of novel 3D cryo-based CLEM methodologies developed at Janelia Research Campus, which are freely available through the Advanced Imaging Center.
Jesse Aaron
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