Centre for Cell Imaging

The Liverpool Centre for Cell Imaging (CCI) is a world class resource for imaging projects. Since 2002, it has been housed in a state-of-the-art suite of custom-designed temperature controlled rooms in the Biosciences Building (Institute of Integrative Biology). We specialise in a range of microscopy techniques including confocal, epifluorescent and lightsheet microscopy as well as having expertise in photothermal imaging and post-acquisition analysis.



Expertise

Imaging Platforms

Keywords - Light Microscopy: Confocal microscopy | Superresolution microscopy | Widefield microscopy | TIRF | Live cell imaging | Multi-photon microscopy | FCS/FCCS | Atomic Force Microscopy | Light-sheet microscopy (SPIM) | Time-domain FLIM; Photothermal

-Microscope 1: Zeiss 880 multiphoton confocal microscope 
-Microscope 2: Light Sheet Z.1 (Zeiss)
-Microscope 3: Zeiss LSM 710
-Microscope 4: Zeiss LSM780 equipped with a PicoQuant FLIM system for FLIM, phosphorescence FLIM, FLIM-FRET, FCS, RICS and FCCS, 
-Microscope 5: Fully motorised inverted Wide-field Zeiss Axio ObserverZ1, equipped with Andor iXon ULTRA High speed EMCCD camera for SOFI
-Microscope 6: Zeiss 880 BioAFM: hybrid confocal equipped with a JPK AFM and Zeiss TIRF
-Microscope 7: Histology workstation
Zeiss Axiovert microscope fitted with a Leica DFC450 C
-Microscope 8: Multiphoton Zeiss LSM 510 
-Research microscopes 9 & 10: Photothermal 1 & 2 for materials gold and other non luminescent nanoparticles in cells
-Research Microscope 11: Atomic Force Microscope Multimode 8 (Bruker)
-Micro-injection station (Narishige), for microscope 5

Applications

Keywords – Biological: Cell Biology | Neurobiology | Developmental Biology | Immunobiology | Plant Biology | Zebrafish | C.elegans | Drosophila | Microbiology | Bio-materials | Histology/pathology | Food
Keywords - Physical Sciences: Bioengineering and biomaterials |Nanomaterials (nanoparticles, 2D nanomaterials, nanowires and nanotubes) | Pharmacology

As a Technology Directorate Shared Research Facility on campus, the CCI has been designed to cater for a wide variety of applications, for academy and industry alike.The Technology Directorate is an organisation within the University of Liverpool Faculty of Health and Life Sciences that promotes access to enabling technology and the academic expertise that supports it.

Sample Preparation

Keywords - Biological: Cryosectioning | Immunolabelling | Plunge Freezing

We are happy to help users to find the best way to prepare their own samples. We can accommodate a variety of samples, ranging from simple slides to multi-well plates and samples embedded in 3D matrices for the light sheet.

Data Analysis

Keywords - Software: ImageJ |FIJI | Imaris | Matlab | Volocity | micromanager | Zeiss ZEN and LSM510
Keywords - Hardware: 3 workstations with at least 64GB RAm each/ Upgraded data storage: Server 4U Dual Socket Intel -24 bays SAS/SATA, 140TB with mirror for selected data, OS Red Hat, hosting the OMERO software (VA technologies)

Modern biological imaging produces vast amounts of data. At the CCI we have the infrastructure and expertise to be able to support users in everything from short-term storage of a few gigabytes to the long-term storage of terabytes. All of which can be made accessible on or off-site.
We have a suite of dedicated workstations, running a range of software packages (such as Fiji, Matlab and Imaris) that can be used for image analysis and processing, and a full-time Image Analyst.
The Liverpool CCI is proud to have adopted OMERO to facilitate records management and storage. This server-based system greatly facilitates the storage, access (public or private) and archival of imaging data.

Shared Access

The Centre for Cell Imaging was established as a Shared Research Facility (SRF) in May 2011. An SRF is defined as open access and is under the umbrella of the Faculty's Technology Directorate. The Technology Directorate was set up by the Faculty in response to the Wakeham report and is led by Prof Rob Beynon. It is an independent Department, funded directly by Faculty. The key policies are: open access (all users have equal rights), which does not equate to free access.

Funding

The CCI is under the umbrella of the Technology Directorate. Amongst the last grants, MRC (clinical infrastructures - Next generation microscopy), BBSRC (Alert 13 and Alert 14).



Share this