Aberdeen University Microscopy and Histology Core Facility

The Microscopy and Histology Core Facility is based in the Institute of Medical Sciences at the University of Aberdeen. We are pleased to welcome all users with all levels of experience in microscopy and/or histology from the university and also visiting scientists from other faculties, universities and hospitals. We have a wide variety of equipment available for use and staff members with many years of experience who are happy to help.



Expertise

Imaging Platforms

Keywords - Light Microscopy: Confocal Microscopy | Widefield Microscopy | Laser Microdissection | Live Cell Imaging
Keywords - Electron Microscopy: Transmission EM | Electron Tomography | Scanning EM (Tungsten)
Keywords - X-Ray Microscopy: MicroCT

Confocal microscopy

- Zeiss 700 Laser Scanning Confocal Microscope
- Zeiss 710 Laser Scanning Confocal Microscope

Fluorescence microscopy 
- Zeiss Imager M2 + Apotome
- Zeiss Observer Z1
- Zeiss PALM Laser Microdissection system

Slide Scanner
- Zeiss Axioscan Z1

Micro CT 
- SkyScan-1072 High-resolution desk-top micro-CT system

Live cell Imaging
- Delta Vision Core wide field imaging system with EMCCD
- Perkin Elmer Ultraview Spinning Disk

Histology 

- Tissue processors, standard and motorized microtomes for wax and resin sectioning
- Cryostat for frozen sectioning
- Leica Bond-max fully automated immunostainer

Electron Microscopy 
- JEOL 1400 plus TEM and XR16M-ActiveVu camera
- Zeiss EVO MA10 SEM with Oxford Inca microanalysis
- Leica UC6, UC7 R2 ultramicrotomes
- Cryo Electron Microscopy 
- Leica Empact 2 High Pressure Freezer (with rapid transfer system)
- Leica AFS (Automated Freeze Substitution) processor
- Leica FC6 cryo-ultramicrotome

Applications

Keywords - Biological: Cell Biology | Neurobiology | Developmental Biology | Plant Biology | Zebrafish | C.elegans | Microbiology | Histology/Pathology | Food
Keywords - Physical: Geology/Mineralogy

As a busy multi-user facility, we see a wide variety of samples passing through. We have the expertise to deal with many different sample types.

Sample Preparation

Keywords - Biological: Resin Embedding | Ultrathin Sectioning | Serial Sectioning | Tokuyasu Embedding | Cryosectioning | Immunolabelling | High Pressure Freezing | Freeze Substitution | Critical Point Drying | Metal Coating | Negative Stain | Correlative Light and EM | Immunofluorescence | Cryostat Sectioning | H&E Staining | Enzyme Histochemistry | Live Cell

We are able to advise and assist users on the best way to prepare their samples.

Data Analysis

Keywords - Software: ImageJ | FIJI | Imaris | Amira | IMOD | Volocity | ImagePro | AxioVision

We can support users in the analysis of their data and there is a range of software packages available for use. Training can be given if required.



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