Advanced BioImaging Facility

The ABIF has been in operation for over 15 years at the McGill University Life Sciences Complex providing guidance to researchers with their projects from sample preparation to data analysis. Comprehensive consultations, sample preparation advice, ongoing support, image processing, image analysis and preparation of data for publication all contribute to using researcher’s time effectively, generating high quality quantitative data and elevating research projects to the next level.


Imaging Platforms 

Keywords - Light Microscopy: Superresolution microscopy | Laser microdissection | Live cell imaging | TCSPC-FLIM | Light-sheet microscopy (SPIM) | Confocal microscopy | Widefield microscopy | TIRF,Multi-photon microscopy | FCS/FCCS | Structured Illumination Microscopy 
Keywords - Medical: in vivo fluorescence imaging

The ABIF offers more than twenty state-of-the-art light microscopy and image analysis platforms.


Keywords - Biological: Cell Biology | Developmental Biology | Plant Biology | C.elegans | Microbiology | Histology/Pathology | Neurobiology | Immunobiology | Zebrafish | Drosophila | Bio-materials 
Keywords - Physical: Bioengineering & Biomaterials 

The facility has expertise in many areas of cellular imaging including, but not limited to, live cell imaging, FRAP, FLIM, FRET, FCS, image correlation spectroscopy, TIRF, spectral imaging, high content screening, multi-photon imaging, laser capture micro-dissection, cellular image analysis, and custom image analysis.

Sample Preparation

Keywords - Biological: Immunofluorescence | Live cell
Keywords - Material: Tissue clearing

The ABIF can provide advice on biological sample preparation with expertise in immunofluorescence or live imaging. We provide a platform for tissue clearing with X-Clarity. Many of our platforms are equipped with temperature and CO2 control for live cell imaging. 

Data Analysis 

Keywords - Software: ImageJ | Imaris | Fiji | Matlab | AutoQuant |Metamorph
Keywords - Hardware: Zen | Image-Pro Premier | OriginPro | SIMFCS

Our experts in Image Analysis can provide one-on-one training tailored to specific needs of the user covering multiple aspects of image analysis. Also, our free consultation will help users design experiments with proper tools required for subsequent image analysis.

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