Advanced Imaging and Cytometry Facility

The Advanced Imaging and Cytometry Facility’s remit is to provide state of the art tools for both the fundamental and translational study of cancer (from molecular interactions in primary cells through to tissue-wide responses), to introduce and develop new modalities, and to train scientists to apply these techniques to their research. Founded in 2001 the laboratories contain an array of equipment some of which is GLP compliant.



Expertise

Imaging Platforms

Keywords - Light Microscopy: Confocal microscopy | Superresolution microscopy | Widefield microscopy | Laser microdissection | Live cell imaging | Multi-photon microscopy |Gated-STED | Flow Cytometry Sorting | Flow Cytometry analysis | Imaging Cytometry | High Content Screening | Confocal High Content Screening | High Throughput Screening | Tissue Screening | Whole slide imaging | spectrophotometric imaging
Keywords - Medical Imaging: In vivo fluorescence imaging | Photoacoustic imaging | Ultrasound

The facility has a range of microscopy and cytometry techniques for fundamental oncology research, in addition to an array of high content and high throughput systems suitable for translational visualisation and analysis.

Applications

Keywords – Biological: Cell Biology | Immunobiology | Bio-materials | Histology/pathology | Oncology

The concern of the facility is the visualisation and mathematical modelling of oncology, from single molecule, through to single cell, micro-tissues, tissues to system wide approaches.

Sample Preparation

Sample preparation is provided by the Institute's Histology facility or directly from the laboratories onsite at the Cancer Research UK: Manchester Institute or the Manchester Cancer Research Centre.

Data Analysis

Keywords - Software: ImageJ | FIJI | Imaris | Matlab | Huygens | ImagePro | Metamorph | AxioVision | Definiens | Harmony | Columbus | Inform | R | LAS

A facility shared IT space for the analysis of data is provided with four workstations. The Institute's Scientific Computing department provide 700TB of temporary storage for imaging. Long term tape backup is available. Some of the software available to the researcher and clinician is web enabled for access outside of the laboratory.

Shared Access

A limited amount of time is available, all applications are to made to the head of the facility and undergo a review process.

Funding

All funding is directly from Cancer Research UK. Super-Resolution equipment has been funded by the Manchester Cancer Research Centre.



Share this