Bristol University Wolfson Bioimaging Facility

The Wolfson Bioimaging Facility provides microscopy support for researchers across Bristol University and beyond. A broad range of fluorescence imaging systems are positioned alongside high specification electron microscopes. Expert support is provided for preparation, imaging and analysis. The fully integrated nature of the facility allows us to combine multiple imaging modalities, including correlative microscopy.



Expertise

Imaging Platforms

Keywords - Light Microscopy: Cryo-fluorescence | Superresolution microscopy | Live Cell Imaging | Gated-STED | TCSPC-FLIM | Light-sheet microscopy (SPIM) | Confocal microscopy | Widefield microscopy | TIRF | Multi-photon microscopy | FCS/FCCS | High content screening | Deconvolution microscopy
Keywords – Electron Microscopy: Transmission EM | Scanning Transmission EM | Electron tomography | Scanning EM (FEG) | Cryo-TEM | Cryo-ET | Serial Block Face SEM | Integrated Light and TEM | Scanning EM (LaB6)

An extensive range of advanced fluorescence imaging systems (including confocal, wide-field, multiphoton, super-resolution, TIRF, FLIM, lightsheet and HCS) are positioned alongside high specification electron microscopes (TEM, SEM, SBF-SEM ) to provide exceptional opportunities for microscopy applications.

Applications

Keywords – Physical Sciences: Bioengineering and biomaterials | Nanomaterials 

Sample Preparation

Keywords – Biological: Resin embedding | Ultrathin sectioning | Serial sectioning | Tokuyasu embedding | Cryosectioning | Immunolabelling | Plunge Freezing | High Pressure Freezing | Freeze substitution | CEMOVIS | Critical Point Drying | Negative stain | Photoconversion | Correlative Light and EM | PLT | Immunofluorescence | Live Cell

Data Analysis

Keywords - Software: ImageJ | Imaris | IMOD | Huygens | FLIMfit | Fiji | Amira | Matlab | Velocity | Metamorph

A data analysis suite provides access to commercial and open-source image processing and analysis tools. A dedicated image analyst provides advice, training and development of bespoke analysis tools.

Shared Acces

The Facility is open for outside business based on capacity. We operate on a Full Economic Costing recovery basis.

Funding

The Facility was formed in 2008 by the merger of the MRC Cell Imaging Facility (established 1996) with EM facilities in the Department of Physiology and the acquisition of new laboratory space and equipment (LM and EM systems) funded by the Wolfson Foundation and University of Bristol. Subsequently the Facility has grown rapidly with addition of >10 microscope systems funded by MRC, BBSRC, Wellcome Trust, EPSRC and University sources. In 2016 the facility expanded into a new microscopy suite funded by the Wolfson Foundation.



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