Central Biotechnology Services

Central Biotechnology Services is an ISO 9001:2015 and GCLP certified Cardiff University Technology Facility that provides genomics and bioinformatics, proteins and diagnostics and cell analysis/imaging platforms, services, training and expertise to local researchers and external organisations. They also host companies and provide laboratory facilities, technological support, academic consultancy and access to other university resources.



Expertise

Imaging Platforms

Keywords - Light Microscopy: Confocal microscopy | Superresolution microscopy | Live cell imaging

Keywords - Electron Microscopy: Transmission EM | Scanning Transmission EM

Keywords - Flow Cytometry: Analytical Flow Cytometry | Cell sorting | Imaging Flow Cytometry 

Electron microscopy - Routine and specialist wax and resin embedding with full range sectioning capability, routine and specialist staining (including immunohistochemistry), analytic scanning and transmission EM, critical point drying and sputter coating, nanoparticle isolation and characterisation, methodological development with notable expertise in bespoke tissue processing. Specific expertise with JOEL 840A SEM and FE1 CM12 TEM.

Further imaging capabilities:
Range of light and fluorescence microscopy available for mineral and biological samples. X-ray imaging, bone density calculations, fluorescence and bioluminescence imaging using Bruker Fx-Pro and PerkinElmer IVIS 200 for in vivo and ex vivo macro samples. Super-resolution microscopy using a Zeiss ELYRA. 2 x FACS Canto II, 1 x LSRFortessa, 1 x Cyan ADP, 1 x Attune NxT, 1 x Imagestream Mark II, 1 x Aria III cell sorter.

Applications

Keywords - Applications: Phenotyping | Cell cycle | Mammalian cells | Fluorescent proteins 

Software

CBS hold a site licence for FlowJo which allows users to obtain an individual FlowJo licence for a reduced fee 



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